Detection of Genetic Markers of Lung Cancer Initiation and Progression
University of Pittsburgh
Summary
The purpose of this research study is to determine the genetic changes and immunologic changes that are involved in the development and progression of bronchogenic lung cancer.
Description
The multistage theory of carcinogenesis includes the development of multiple activating genetic changes due to exposure to carcinogens, either primarily, or superimposed upon pre-existing mutations in the genome. These changes result in activation of protooncogenes, lack of expression of tumor suppressor genes, or combinations of the above, the sum of which results in malignant transformation. Detailed analyses of chromosomal lesions in bronchogenic lung cancer reveal several recurring abnormalities, including deletions, duplications or polysomy of chromosomes 1, 3, 7 and 20. Aberrations in th…
Eligibility
- Age range
- 18+ years
- Sex
- All
- Healthy volunteers
- No
Inclusion Criteria: * Histologic confirmation of lung cancer, lung metastases from a primary site other than lung, mesothelioma or a radiographic lesion highly suspicious for malignancy * Written informed consent. * To be scheduled for a biopsy or surgical resection or have already had a biopsy and/or surgical removal of a lung mass Exclusion Criteria: None
Interventions
- ProcedureBiopsy of the major carinal area
Biopsy performed intraoperatively
- ProcedureBiopsy of abnormal & suspicious areas of the bronchial tree
Biopsy performed intraoperatively
- ProcedureEvaluation of the tumor for DNA mutations
Tumor tissue is banked for future analysis.
- ProcedureBronchoalveolar Lavage (BAL) for cytokine analysis
BAL performed intraoperatively
- ProcedureCorrelation of flow cytometric & RT PCR for TNM stage
Tissues banked for future correlative studies
- ProcedureAnalysis of lymph nodes
Tissues banked for future analysis
Location
- Hillman Cancer CenterPittsburgh, Pennsylvania