Effects of Treatments on the Microbiome in Healthy Volunteers and Patients With Atopic Dermatitis
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Summary
Background: \- Atopic dermatitis, or eczema, is a chronic skin disorder. Patients sometimes have infections with S. aureus bacteria. Researchers want to study how eczema treatments affect the number and the type of bacteria on the skin. Objectives: \- To study the effect of eczema treatments on skin bacteria. Eligibility: * Individuals between 2 and 25 years of age who have moderate to severe atopic dermatitis. * Healthy volunteers between 18 and 40 years of age with no history of eczema. Design: * Participants will be screened with a physical exam and medical history. Research samples will be collected. Skin biopsies may also be performed. * All participants will be assigned to one of several study groups. * Healthy volunteers must not have taken antibiotics in the year before the start of the study. * All participants will have regular study visits during their 1-year participation. More research samples will be collected at these visits. * Healthy volunteers may be asked to come in for a one-time follow up after the 1 year mark.
Description
BACKGROUND: * The use of antibiotics has revolutionized medicine, yet the impact of antimicrobials on the human microbiome is incompletely understood. * Antimicrobial treatments, including topical and systemic antibiotics, are highly effective and are frequently used to manage disease flares of atopic dermatitis (AD). Concomitant use of dilute bleach baths reduces the clinical severity of AD in patients with clinical signs of bacterial skin infections. * The longitudinal impact of various antimicrobials on the human microbiome, particularly in skin, has not been systematically investigated. *…
Eligibility
- Age range
- 2–50 years
- Sex
- All
- Healthy volunteers
- Yes
* INCLUSION CRITERIA: Cohorts 1 \[NO FURTHER ACCRUAL\], 2 \[INACTIVE\], 4 \[NO FURTHER ACCRUAL\] and 5 \[INACTIVE\]: Healthy Volunteers * Males and females aged 18-50 years at time of initial protocol sampling * Subjects must participate fully and be willing to comply with the procedures of the protocol * Subjects must be co-enrolled in NIH protocol 08-HG-0059 * Ability of subject to understand and provide written informed consent * Access to bathing facilities (Cohort 2 \[INACTIVE\]) * Ability to swallow capsules or tablets Cohort 3 \[INACTIVE\]: Atopic Dermatitis Patients * Subjects must…
Interventions
- DrugTrimethoprim/sulfamethoxazole (TMP/SMZ)
800/160 orally every 12 hours for 14 days
- DrugCephalexin
500 mg orally every 8 hours for 14 days
- DrugDoxycycline
20 mg orally every 12 hours for 56 days or 100 mg orally every 12 hours for 56 days
- OtherSodium hypochlorite
6 % dilute bleach
- OtherPlacebo capsules
Capsule orally every 8 hours daily for 14 days
- OtherPlacebo Sodium hypochlorite
Three times a week for 14 days
Location
- National Institutes of Health Clinical CenterBethesda, Maryland