Nasal Endotoxin Challenge to Study Mucosal Inflammation in Patients With Asthma
National Jewish Health
Summary
A phase I clinical research study aimed at determining mechanisms that regulate airway mucosal inflammation in asthma endotypes using intranasal administration of endotoxin (lipopolysaccharide from E. coli) in healthy controls and subjects diagnosed with asthma.
Description
Patients with severe asthma can be broadly classified into three endotypes (T2, T1, and T17) based on inflammatory cell and cytokine profiles. Within these endotypes, many patients have high levels of neutrophils in the airways and mucosal epithelium. Our preliminary data suggest that neutrophils in the airways and blood of patients with severe asthma are heterogenous and that subsets exist. The nature of these subsets appears to differ between T2, T1, and T17 asthma endotypes. In order to advance the field and determine the mechanisms that underpin severe neutrophilic asthma, investigators p…
Eligibility
- Age range
- 18+ years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: * Participants will have asthma diagnosed by a health care provider. Healthy controls are individuals without asthma. * Written informed consent Exclusion Criteria: * Current or recent illness (in the past 4 weeks) * Recent asthma exacerbation (past 4 weeks) * History of nasal perforation or nasal surgery * Nasal polyposis * Presence or prior history of cardiac or systemic disease * Bleeding disorder, use of systemic anticoagulants, or antiplatelet therapy * Immunocompromised state (HIV, immunoglobulin deficiency, systemic immunosuppressants excluding corticosteroids) *…
Interventions
- DrugLipopolysaccharides
LPS aka endotoxin is a component of bacterial cell walls and is ubiquitous in the environment. It is found in pet dander, house dust and environmental dusts and has been heavily studied in asthma.
Location
- National Jewish HealthDenver, Colorado