Circadian Regulation of Human Adipose Tissue Metabolism
Mayo Clinic
Summary
The goal of this study is to understand the underlying circadian rhythms in subcutaneous adipose tissue of humans with our without continuous feeding and how these are altered in people who have obesity.
Description
This proposal will address 3 main aims: 1) Determine the impact of endogenous AT circadian rhythms on AT function independent of diurnal nutrient delivery in humans, 2) determine the impact of central obesity on adipocyte circadian clock, and 3) establish for the first-time in vivo human adipocyte specific cistrome of the core circadian clock transcription factors, BMAL1 and CLOCK. In order to understand whether adipocytes have intrinsic circadian clock entrainment that is separate from food entrainment, we will compare rhythmicity of AT of lean volunteers studied under continuous vs. intermit…
Eligibility
- Age range
- 18–55 years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: * BMI 20-25 kg/m2 or 30-37 kg/m2 * For participants with obesity a waist-to-hip ratio of ≥0.95 in males and ≥0.90 in females. * sedentary * females: non pregnant or breastfeeding * ability to provide written informed consent and follow study instructions Exclusion Criteria: * History of mediations that impact adipocyte/lipid metabolism * smoking * insomnia * sleep apnea * sleep medication use * employment in night or shift work * extreme chronotypes * Allergy to lidocaine
Interventions
- OtherContinuous Feeding
Participants will receive 24-hour continuous feeds through an NG tube
- OtherIntermittent feeding
Participants will receive 3 boluses of feeding per day at regular meal times through an NG tube to mimic normal meals.
Location
- Mayo ClinicRochester, Minnesota