MRS Measurement of Glutamate and GABA Metabolism in Brain
National Institute of Mental Health (NIMH)
Summary
This study will use magnetic resonance spectroscopy (MRS) to measure in the brain the transfer of \[13\]C as it is naturally metabolized from glucose to specific chemical transmitters. From this method, we can measure the rate of production of an important excitatory neurotransmitter (glutamate) as well as an inhibitory neurotransmitter (GABA).
Description
13C is a stable (i.e., non-radioactive) isotope of carbon with a natural abundance of \~1%. Following infusion of \[13C\]glucose and/or \[13C\]acetate, in vivo MRS (magnetic resonance spectroscopy) can monitor the rate of flux of the 13C atom from glucose and/or acetate to glutamate to glutamine. Thus, this procedure can provide measure of glutamate (GLU) and glutamine (GLN) turnover in brain. We have established parameters to obtain these measurements in nonhuman primate brain. The current protocol seeks approval to optimize MRS parameters and to develop new MRS techniques for human brain usi…
Eligibility
- Age range
- 18–65 years
- Sex
- All
- Healthy volunteers
- Yes
* INCLUSION CRITERIA: * 18-65 years of age * Able to give written informed consent * Healthy based on medical history and physical exam * Enrolled in Protocol 01-M-0254 or Protocol 17-M-0181 EXCLUSION CRITERIA: * Any current Axis 1 diagnosis * Clinically significant laboratory abnormalities * Positive HIV test * Metallic foreign bodies that would be affected by the magnetic resonance imaging (MRI) magnet, or fear of enclosed spaces likely to make the subject unable to undergo an MRI scan. * History of neurological illness or injury with the potential to affect study data interpretation, such…
Interventions
- Device3T and 7T device
3T and 7T device
Location
- National Institutes of Health Clinical CenterBethesda, Maryland