Mechanisms Underlying Impaired Wound Healing
University of Pittsburgh
Summary
The overall aim of this research is to utilize wound derived inflammatory cells from diabetic versus non diabetic human chronic wounds to understand mechanisms that are responsible for disregulated inflammation in individuals with diabetes. Biology of normal (peripheral blood derived cells) versus wound derived cells will also be studied.
Description
Specific aims: 1. To study biology of wound derived inflammatory cells and fluid from diabetic versus non diabetic wounds. 2. To characterize mechanisms that are responsible for dysregulated wound inflammatory cell function in individuals with diabetes. 3. To characterize differences in the biology of normal peripheral blood derived cells versus wound derived cells. The long-term goal of this study is to determine the molecular mechanisms underlying impaired wound healing in diabetics.
Eligibility
- Age range
- 21–80 years
- Sex
- All
- Healthy volunteers
- No
Inclusion Criteria: * Ages 21-80 * Chronic Wound (present \>4 weeks) * Diabetic \& Non-diabetic * Adult surgical wounds Exclusion Criteria: * Inability to consent * Pregnant women * Therapeutically immunocompromised * Individuals defined in 45 CFR 46 Subparts B,C, and D, nor from any other population considered vulnerable 2.9
Interventions
- OtherBlood draw
Blood will be drawn from subjects once and VAC sponge will be collected (which is normally discarded material per standard of care).
Locations (2)
- UPMC MontefiorePittsburgh, Pennsylvania
- UPMC PresbyterianPittsburgh, Pennsylvania