Reversal of Lipid-Induced Insulin Resistance
Yale University
Summary
The purpose of this study is to examine whether weight reduction decreases intramyocellular (IMCL) and hepatic lipid content, and improves insulin sensitivity of muscle and fat tissue in people who are insulin resistant and have a family history of type 2 diabetes. Hepatic mitochondrial oxidation will be assesses using a 3 hour triple tracer study (D7 glucose, 3-13C lactate and 13C4 beta-hydroxybutyrate).
Description
In this study, we will examine whether a small weight loss in lean, insulin-resistant offspring of type 2 diabetic patients will improve insulin resistance. The control group will consist of subjects matched for gender, age and body weight with no family history of diabetes. Before and after weight loss, rates of basal and insulin stimulated whole body glucose metabolism will be measured using \[6,6-2H\] glucose during a 3 hour basal period and a 4 hour euglycemic hyperinsulinemic (20 mU/m2-min) clamp. Rates of whole body lipolysis will be determined using \[2H5\] glycerol, localized rates of…
Eligibility
- Age range
- 18–90 years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: * Healthy, sedentary, non-smoking and not taking any medications other than birth control pills. * Hematocrit \>35% * Subjects will have no systemic or organ disease including diabetes. * Subjects will have no history eating disorders. * Women must be using a form of birth control (sexual abstinence, birth control pills, Norplant, IUD or condoms) and will be studied between day 0 and 7 of their menstrual cycle. * Those who are taking birth control pills or have had a hysterectomy may be studied at any time. * Physical activity will be assessed using a standard questionnair…
Interventions
- BehavioralCaloric Restriction
Dietary intervention to reduce lipid content in liver and muscle with minimal overall weight loss. Weekly visits for measurements of body weight, body composition, blood glucose and MRS measurements of liver and muscle lipid.
Locations (2)
- Magnetic Resonance Research Center (MRRC)New Haven, Connecticut
- Yale Center for Clinical Investigation HRUNew Haven, Connecticut