In Vivo Assessment of Glutamine Utilization by Bone Marrow Plasma Cells in Healthy Subjects Using Stable Isotope Resolved Metabolomics: A Pilot Study
Mayo Clinic
Summary
This pilot study will first establish the feasibility of an in vivo methodology of assessing the utilization of glutamine into the TCA cycle of normal bone marrow plasma cells from healthy subjects while comparing it to an ex vivo approach
Description
Twenty healthy adult study participants between the ages of 18 and 60 years will be recruited to participate in this pilot study that will include two groups: a) Ex Vivo group (10 volunteers) and b) In Vivo group (10 volunteers). Volunteers will be recruited to either group in a sequential alternating fashion. In vivo 13C SIRM studies evaluating the utilization of glutamine into the TCA cycle of normal bone marrow plasma cells.
Eligibility
- Age range
- 18–60 years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: * Ages 18-60 * Able to provide written consent Exclusion Criteria: * Renal Failure * Pregnancy * Active steroid use * Active liver Disease * Anemia (Hemoglobin \< 12.5 g/dL in men and \< 11.5 g/dL in women) * H/O alcohol use (average \> 2 drinks per day) * BMI \> 30
Interventions
- Other5-13C-Glutamine
Intravenous infusion of 5-13C-Glutamine
- OtherBone marrow aspiration
Bone marrow aspiration of bone marrow aspirate
Location
- Mayo ClinicRochester, Minnesota