Metabolic Inflexibility is Related to Elevated Muscle Anaerobic Glycolysis
East Carolina University
Summary
The focus of this proposal is on overweight (25\>BMI\<30 kg/m2) subjects, as these individuals exhibit a high risk of becoming obese and/or developing metabolic diseases. We hypothesize that in some overweight individuals there is a "metabolic program" in skeletal muscle which predisposes them to the development of obesity. Findings may lead to clinical screening tools for determining risk for obesity in non-obese individuals and targeting this group for prevention.
Description
Our overall hypothesis is that increased reliance on anaerobic glycolysis in muscle shifts fasting metabolism from fat towards carbohydrate utilization, which results in metabolic inflexibility and subsequent metabolic disease (i.e. obesity). To test our hypothesis, overweight subjects (BMI 25 to 30 kg/m2) will be recruited and screened for reliance on anaerobic glycolysis (resting/fasting plasma lactate concentrations). Subjects with high (top quartile) and low (bottom quartile) resting/fasting plasma lactate will be chosen. The premise of this screening is that subjects with low lactate will…
Eligibility
- Age range
- 18–50 years
- Sex
- All
- Healthy volunteers
- No
Inclusion Criteria: * 18-50 years old * BMI of 25 - 30 kg/m2 * BMI \> 40kg/m2 * Lactate levels in top and lower 25% Exclusion Criteria: * Pregnant women * Mentally disabled * Prisoners * Smokers * Subjects with heart disease * Type 1 and 2 diabetes * Endocrine disease * Hypertension * Musculoskeletal disease * Peripheral occlusion * Hepatic disease * Have had weight fluctuations exceeding + 3% in the previous 12 months * On medications which alter carbohydrate metabolism will not be studied
Interventions
- ProcedureWeight loss surgery
Severely obese women (BMI \>40) undergo sleeve gastrectomy
Location
- East Carolina UniversityGreenville, North Carolina