Oxidative Lipidomic Biomarkers During Mechanical Ventilation in Critically Ill Patients (OXY-BAL).
Ohio State University
Summary
The aim of this study is to identify and determine the levels of oxidized lipids and lipid mediators following exposure to oxygen supplementation during mechanical ventilation by oxidative lipidomics. The investigators will include patients with mechanical ventilation and have received FiO2=\>0.5 atleast 90 minutes and collected two sequential mini bronchoalveolar lavage on them 24 hours apart. Mass Spectrometry Lipid chromatography will be conducted and clinical data will be analyzed.
Description
This proposal will elucidate an approach for precise and safe oxygenation levels and correlation with the oxidative lipid biomarker profile of optimal oxygenation. The aim of this study is to identify and determine the levels of oxidized lipids and lipid mediators following exposure to oxygen supplementation during mechanical ventilation by oxidative lipidomics. Alveolar cellular membrane and lung lining fluid are the primary targets of therapeutic oxygen and comprised of proteins, cholesterol, and lipids. Among these components, lipids are more susceptible to oxidation producing downstream me…
Eligibility
- Age range
- 18–110 years
- Sex
- All
- Healthy volunteers
- No
Inclusion Criteria: • Adults age =\> 18 and needing Mechanical ventilation in Intensive Care unit expected atleast for 48 hours Exclusion Criteria: * 1.Mechanical ventilation, only needed for performing procedures- bronchoscopy, esophagogastroduodenoscopy, colonoscopy, or others * 2)Chronic respiratory failure prior to admission; * 3)Hemoptysis or diffuse alveolar hemorrhage * 4)Medication use such as N acetyl cysteine; aspirin, fish oil supplements. * 5)Primary team prohibits bronchoscopy for a specific clinical indication or safety;
Interventions
- Otheroxygen
oxygen exposure of more than 50% for 1.5 hours
Location
- Ohio State University Medical CenterColumbus, Ohio