Role of Autophagy in Type 2 Diabetes Microvascular Dysfunction
Medical College of Wisconsin
Summary
The goal of this clinical trial is to examine the role of autophagy on microvascular function in adults with Type 2 Diabetes. The main question it aims to answer are: * Does presence of Type 2 Diabetes reduce autophagy and impair microvascular function? * Does exposure to high glucose impair autophagy and subsequently microvascular function? Participants will undergo 2 study days. The primary outcome will be in vivo microvascular function testing. Following the first study day participants will undergo either supplementation with trehalose, an autophagy activator, or placebo for 14 days. The second study day will test in vivo microvascular function.
Description
It is generally accepted that endothelial dysfunction precedes overt cardiovascular disease and is a foundational event in development of atherosclerosis. Endothelial function is often assessed by flow-mediated dilation (FMD), a physiological response to shear stress along the endothelium that elicits vasodilation via release of nitric oxide (NO) in large and small blood vessels. In the presence of Coronary Artery Disease (CAD), NO production is reduced and FMD is diminished in conduit arteries. However, in the microcirculation, FMD is preserved but there is a compensatory shift from NO to hyd…
Eligibility
- Age range
- 18–80 years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: Participants must be between 18-80 yrs. of age and will be delineated into two groups * Clinical Diagnosis of Type 2 Diabetes Mellitus in accordance with American Diabetes Association (ADA) guidelines (Type 2 Diabetic group) * Otherwise healthy individuals (Healthy control group) with no more than 1 cardiovascular risk factor Exclusion Criteria: * Uncontrolled hypertension * Current Tobacco use or within last 6 months * BMI \> 35 * Hyperlipidemia * Hypercholesterolemia * Type 1 Diabetes * Use of anti-coagulant drugs, or anti-platelet drugs * Symptomatic coronary artery d…
Interventions
- DrugTrehalose
Trehalose will be given at a dose of 10g mixed in 500 mL of water to be consumed 1 time per day for 14 days
- DrugPlacebo
Microcrystalline cellulose will be given at a dose of 10g mixed in 500 mL of water to be consumed 1 time per day for 14 days
Location
- Medical College of WisconsinMilwaukee, Wisconsin