Cell-free DNA Epigenomics to Track the Dynamics of Organ Damage and Immune Exhaustion During Sepsis
Mayo Clinic
Summary
This is a prospective, non-randomized study investigating if organ damage and immune changes can be measured by liquid biopsy NGS through advanced analytical methods.
Description
To determine the sample size needed to derive a signature matrix to identify novel biomarkers in sepsis, investigators performed a mathematical modeling exercise of the lower limits of sensitivity of this liquid biopsy approach. The detection limit of cell-free nucleic acid analysis is determined in part by the number of independent transcripts or "reporters" that are interrogated. Using an established and independently validated binomial model that was previously applied to the prediction of circulating tumor nucleic acid detection limits, investigators estimated the probability of cell-free…
Eligibility
- Age range
- 18+ years
- Sex
- All
- Healthy volunteers
- No
Inclusion Criteria: • Patients who've developed a shocked state (Hypotension or Lactate grade \>4) or Clinical Concern Exclusion Criteria: * \<18 years of age * Pregnant Women * Nursing women
Interventions
- OtherObservation
One additional blood draw in the Emergency Department as part of their standard of care visit.
Location
- Mayo Clinic RochesterRochester, Minnesota