The Effects of Woodsmoke Exposure on Nasal Immune Responses to Influenza Infection in Normal Human Volunteers
University of North Carolina, Chapel Hill
Summary
This study will investigate the effects of woodsmoke (WS) exposure on human nasal mucosal immune responses to viral infection. The study tests the hypotheses that WS exposure modifies biomarkers of nasal mucosal immune function, increases in Live Attenuated Influenza Virus (LAIV) -induced nasal symptoms, and reduces mucosal antibody production.
Description
This study will investigate the effects of woodsmoke (WS) exposure on human nasal mucosal immune responses to viral infection. The study tests the hypotheses that WS exposure modifies biomarkers of nasal mucosal immune function, increases LAIV-induced nasal symptoms, and reduces mucosal antibody production. Healthy volunteers will be randomized for a 2-hr exposure to WS or placebo (filtered air) and then inoculated with either live attenuated influenza virus (LAIV) or placebo. Nasal mucosal samples, symptoms, and peripheral blood will be collected on days 1,2,3,7, and 21 post-exposure/LAIV and…
Eligibility
- Age range
- 18–49 years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: * Normal lung function, * oxygen saturation of \>94%, * normal blood pressure, * no respiratory symptoms on history, no abnormalities on exam, normal pulmonary function testing, * 18-49 Years of age. Exclusion Criteria: * A history of significant chronic illnesses (to include diabetes, autoimmune diseases, immunodeficiency state, known ischemic heart disease, chronic respiratory diseases such as chronic obstructive pulmonary disease or asthma, hypertension) * Positive pregnancy test within 48 hours of the time of challenge * Use of any inhaled substance (for medical or r…
Interventions
- BiologicalLAIV nasal vaccine is chosen as a model viral infection
Inoculation with LAIV
- OtherWood smoke
Wood smoke exposure concentrations at 500 ug/m3 for two hours.
- BiologicalPlacebo for LAIV nasal vaccine is chosen as a model viral infection
Placebo for LAIV inoculation. Nasal administration of normal saline.
- OtherPlacebo for Wood Smoke (clean Air Exposure)
Clean Air Exposure for 2 hours
Location
- Mary Ellen JonesChapel Hill, North Carolina