Muscle-derived Extracellular Vesicles and Their Interactions With Adipocytes in Human Metabolic Dysfunction
Yuan Wen
Summary
This study examines how muscle cells communicate with fat cells through tiny packages called extracellular vesicles (EV) during exercise. These vesicles carry important molecules that may affect how the body processes sugar and fat. The research team observed significant variability in the adipose response to exercise, and used this variability to gain further insight into the mechanism through which mature microRNA-1 (miR-1) changes in adipose tissue. The investigators selected six subjects with the highest increase in miR-1 abundance in adipose tissue after exercise and compared them with the six subjects that had the most dramatic decrease in miR-1 abundance after exercise. The research team observed that participants intrinsically vary in their ability to endocytose EV into adipose tissue. It is unclear whether this variance in receptivity is a cause or consequence of the significant difference in EV-delivery of miR-1 to adipose tissue.
Description
This study investigates muscle-derived extracellular vesicle (EV) communication with adipose tissue and how this pathway is altered in pre-diabetes. The investigators will recruit 80 participants (40 euglycemic controls, 40 pre-diabetic) aged 30-55 years, equally distributed by sex. Pre-diabetes will be defined as impaired fasting glucose (100-125 mg/dL), impaired glucose tolerance (2-hour oral glucose tolerance test (OGTT) 140-199 mg/dL), or HbA1C 5.7-6.4%. Following informed consent and medical screening at the Center for Clinical and Translational Sciences, participants will undergo baseli…
Eligibility
- Age range
- 30–55 years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: * Age 30-55 years * Sedentary lifestyle (exercise \<1 day/week for at least 3 months prior to enrollment) * Able to provide informed consent * For Control Group: BMI \< 27 kg/m², normal glucose tolerance, no more than 1 feature of metabolic syndrome * For Prediabetic Group: BMI \> 30 kg/m², at least 3 features of metabolic syndrome including prediabetes (defined as fasting plasma glucose 100-125 mg/dL OR 2-hour post-load glucose on 75g OGTT 140-199 mg/dL OR HbA1C 5.7-6.4%) Exclusion Criteria: * Pregnancy (confirmed by pregnancy test in women of childbearing potential) *…
Interventions
- BehavioralAcute Resistance Exercise
Participants will perform three sets of eight repetitions, with a 90-120 second rest between sets, with a fourth set performed to failure. All resistance exercise will be performed on pneumatic resistance devices (Keiser Sports Health Equipment, Fresno, CA).
Location
- University of KentuckyLexington, Kentucky