Investigating the Role of Follicular Fluid microRNAs in Ovarian Aging and Assisted Reproductive Success
University of Central Florida
Summary
The purpose of this study is to investigate the role of exosomal microRNAs (miRNAs) in follicular fluid (FF) as biomarkers of ovarian aging and predictors of in vitro fertilization (IVF) outcomes. The goal is to identify noninvasive molecular markers that correlate with oocyte quality and reproductive potential, particularly in women of advanced maternal age.
Description
There is a substantial gap in understanding how these miRNAs operate in humans, especially in a non-invasive or minimally invasive context. Specifically: * The role of exosomal miRNAs in follicular fluid (FF) in human ovarian aging is not well defined. * Few studies have directly compared miRNA expression profiles in FF across age groups using high-throughput sequencing. * There is limited evidence linking specific FF miRNAs to oocyte quality or IVF success rates. * No validated non-invasive biomarkers based on FF exosomal miRNA currently exist for predicting IVF outcomes, especially in wome…
Eligibility
- Age range
- 18+ years
- Sex
- Female
- Healthy volunteers
- Not specified
Inclusion Criteria: * Women undergoing IVF treatment due to male factor infertility or nonovarian causes. * Age groups: younger women \<31 years and older women \>38 years. * Regular menstrual cycles. * Willing and able to provide informed consent. Exclusion Criteria: * Minors (under 18 years) and women aged 32-37 years * Diagnosis of polycystic ovarian syndrome (PCOS). * History of recurrent pregnancy loss. * Presence of endometriosis. * Diagnosis of ovarian insufficiency. * Metabolic disorders (e.g., diabetes). * History of ovarian surgery or chemotherapy. * Any condition that might impac…
Interventions
- OtherNo Interventions
No interventions for participants
Location
- University of Central FloridaOrlando, Florida