Splicing-Based Predictive Learning for Individual Chemotherapy Evaluation in Colorectal Cancer (SPLICE)
City of Hope Medical Center
Summary
Colorectal cancer (CRC) remains one of the leading causes of cancer-related mortality worldwide. Although adjuvant chemotherapy improves survival after curative resection, its efficacy varies widely among patients. The absence of reliable predictive biomarkers often leads to overtreatment or undertreatment. This study aims to develop a machine learning-based predictive model for adjuvant chemotherapy response using tumor-derived alternative splicing signatures. By integrating RNA-seq data, splicing isoform and clinical outcomes, this study seeks to identify molecular predictors of treatment response and recurrence risk after surgery.
Description
Colorectal cancer (CRC) remains a major global health burden, with adjuvant chemotherapy representing the standard of care after curative resection. However, patient responses to therapy vary widely, and no validated molecular model currently guides adjuvant treatment selection. Recent studies suggest that aberrant alternative splicing-rather than gene-level expression alone-plays a crucial role in shaping chemotherapy sensitivity and tumor recurrence. Yet, these complex transcriptomic variations are often missed by standard differential expression analyses. The ASPAIRE framework (Alternativ…
Eligibility
- Age range
- 18–80 years
- Sex
- All
- Healthy volunteers
- No
Inclusion Criteria: * Histologically confirmed stage II-III colorectal cancer (TNM classification, 8th edition) * Received standard adjuvant chemotherapy after curative resection * Availability of tumor tissue (FFPE or frozen) before chemotherapy * Sufficient clinical data for outcome analysis (recurrence, survival) * Age 18-80 years Stage Exclusion Criteria: * Inflammatory bowel disease * Inadequate RNA quality or lack of consent
Interventions
- OtherSPLICE
A panel of RNA splicing isoform, whose level is tested in tissue samples derived from the primary tumor.
Location
- City of Hope Medical CenterDuarte, California