Impact of Food Combinations on the Stimulation of Post-exercise Muscle Protein Synthesis in Healthy Adults
University of Illinois at Urbana-Champaign
Summary
In the present semi-crossover randomized design, 15 recreationally active young adults (19-40 y) will receive stable isotope tracer infusions and perform a single bout of resistance exercise. Immediately after exercise, participants will ingest either 1 medium russet potato with skins and 2 ounce-equivalent lean ground beef combination (274 kcals, 19.3 g protein, 5 g fat, 37 g carbohydrates, and 4 g fiber), 2 slices of white bread and beef combination (262 kcals, 19.8 g protein, 6.8 g fat, 28 g carbohydrates, and 1.8 g fiber) or 2 ounce-equivalent beef (93% lean meat/7% fat; 110 kcals, 14.8 g protein, and 5 g fat). Repeated blood and muscle biopsies will be collected to determine whole body leucine kinetics, amino acid concentrations, anabolic signaling and myofibrillar protein synthesis rates during the trials.
Description
1. Preliminary Testing 1.1 Preparations: Participants will arrive for the preliminary testing session after an overnight fast (\~10 h no caloric foods/beverages consumed) and having refrained from exercise for the 72 h prior. Prior to any in-person procedures, participants will be briefed on the procedures and risks associated with participation via reviewing the informed consent document. Participants will be encouraged to ask questions and have their questions answered prior to being asked if they'd like to continue. Confirmation will be documented via signature on an informed consent pr…
Eligibility
- Age range
- 19–40 years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: * Age 19-40 yrs * Pre-menopausal * Recreationally-active * Weight stable for prior 6 months Exclusion Criteria: * Age outside of range (20 - 35 yrs) * Pregnancy * Irregular menstrual cycles (i.e., \~21 day or \~35 day cycle) * Participation in previous research using L-\[1-13C\] leucine and L-\[ring-2H5\] phenylalanine * Participation in other ongoing research that interferes with this study (e.g., conflicting diet, activity interventions, etc.)
Interventions
- BehavioralResistance Exercise
Participants will perform leg press and leg extension immediately prior to ingestion of either potato-beef combination, bread-beef combination, or beef alone.
- Dietary SupplementPotato and Beef
Participants will ingest baked russet potato and cooked ground beef immediately after resistance exercise.
- Dietary SupplementBread and Beef
Participants will ingest white bread and cooked ground beef immediately after resistance exercise.
- Dietary SupplementBeef
Participants will ingest cooked ground beef immediately after resistance exercise.
Locations (2)
- Louise Freer HallUrbana, Illinois
- Louise Freer HallUrbana, Illinois