Effects of an Acid-Active Protease (P24) on Postprandial Aminoacidemia Following a Standardized Mixed-Protein Burger Meal in Healthy Adults
Amplifye
Summary
The purpose of this study is to quantify the acute effects of an acid-active protease enzyme supplement (P24) on postprandial aminoacidemia following consumption of a standardized mixed-protein meal containing both plant- and animal-derived protein sources. Secondary objectives include evaluating the postprandial plasma peptidomic response, characterizing the postprandial hormonal responses (GLP-1, insulin, and GIP), and assessing the postprandial glucose response using standardized capillary blood glucose measurements. The study will also evaluate subjective responses including satiety and hunger and gastrointestinal symptoms.
Description
This study is a randomized, double-blind, placebo-controlled crossover trial designed to evaluate the acute effects of an acid-active protease enzyme supplement (P24) on postprandial amino acid availability after consumption of a standardized mixed-protein meal in healthy adults. Each participant will complete two morning test visits separated by an approximately 7-day washout period and will receive P24 during one visit and placebo during the other visit in randomized order. Each participant will therefore serve as his or her own control. The primary objective is to compare postprandial circ…
Eligibility
- Age range
- 45–75 years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: * Healthy adults aged 45-75 years * BMI 18.5-35 kg/m2 * Able to provide informed consent and comply with study procedure * Willing to consume the standardized mixed-protein test meal Exclusion Criteria: * BMI outside of 18.5-35 kg/m2 * Gastrointestinal disorders or malabsorption syndromes that could affect digestion/absorption (e.g., inflammatory bowel disease, celiac disease) * Diagnosed diabetes * Current use of medications or supplements that materially affect glucose metabolism or digestion (e.g., GLP-1 receptor agonists, chronic digestive enzyme supplementation) * R…
Interventions
- Dietary SupplementProtease enzyme supplement
Participants receive one capsule containing P24, an acid-active protease enzyme preparation, prior to consumption of a standardized mixed-protein burger meal during one study visit. The intervention is administered in a randomized, double-blind, placebo-controlled crossover design and is compared with a matching placebo capsule containing microcrystalline cellulose. Postprandial responses are assessed over 180 minutes following meal consumption.
- OtherPlacebo
Participants receive one matching placebo capsule containing microcrystalline cellulose prior to consumption of a standardized mixed-protein burger meal during one study visit. The placebo is identical in appearance to the active capsule and is administered in a randomized, double-blind, placebo-controlled crossover design. Postprandial responses are assessed over 180 minutes following meal consumption.
Location
- Ragle Human Nutrition CenterDavis, California