Determining Cell- and Spatially-distinct Skeletal Muscle Transcriptional Aberrations With Insulin Resistance
AdventHealth Translational Research Institute
Summary
The purpose of this study is to find new signs or signals in muscle cells that can help us understand when the body isn't responding well to insulin (a condition called insulin resistance).
Description
The primary goal of this research is to look at gene expression changes to insulin stimulation in different cells and areas of skeletal muscle and how they are affected by obesity and insulin resistance. The investigators are studying sedentary men and women between the ages of 30 and 65 years with; either a body mass index (BMI) between 18-25 kg/m2 or 30-40 kg/m2
Eligibility
- Age range
- 30–65 years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: * All Groups 1. 30 to 65 years of age 2. Stable weight (No Gain/Loss of \>10 lbs in 6 months) 3. Sedentary (≤ 1 continuous exercise/week) 4. Non-smoker 5. Resting Blood Pressure ≤ 150mmHg systolic and ≤ 95 mmHg diastolic Group A - Lean individuals with insulin sensitivity (IS-Lean) 1. BMI 18-25 kg/m2 2. Hemoglobin A1c ≤ 5.6% at screening 3. Blood glucose level ≤ 140 mg/dL 2hr after a 75g oral glucose tolerance test Group B - Individuals with obesity and insulin sensitivity (IS-Obesity) 1. BMI 30-40 kg/m2 2. Hemoglobin A1c ≤ 5.6% at screening 3. Blood glucose…
Interventions
- OtherVO2 Max
Aerobic Fitness determined by measuring maximal volume of oxygen consumed (VO2max) during a stationary bicycle exercise test.
- OtherDEXA
DEXA scans will be performed to measure body fay and estimate muscle mass.
- OtherPeripheral Microvascular Reactivity
Post-occlusive reactive hyperemia (POHR) will be measured using Near Infrared Spectroscopy
- OtherSkeletal Muscle Insulin Sensitivity
Skeletal Muscle Insulin Sensitivity will be assessed with a hyperinsulinemic -euglycemic clamp with stable glucose isotope tracers.
- OtherNon-Oxidative Glucose Disposal
Rates of glucose oxidation will be measured with indirect calorimetry during the hyperinsulinemic -euglycemic clamp. Rates of non-oxidized glucose disposal will be calculated by subtracting rates of glucose oxidation from rates of glucose disposal during the hyperinsulinemic -euglycemic clamp.
- Other
Location
- AdventHealth Translational Research InstituteOrlando, Florida