Effects of Flavonoid-rich Foods on Carbonyl Stress
North Carolina Agriculture & Technical State University
Summary
This randomized crossover study will compare low- and high-flavonoid diets in healthy adults to identify flavonoid-derived biomarkers in urine and plasma. Using targeted LC-MS metabolomics, researchers will measure known and novel flavonoid metabolites and evaluate whether a higher flavonoid intake reduces carbonyl stress markers, including reactive carbonyl species (RCS) and advanced glycation end products (AGEs).
Description
Biomarkers of flavonoids and flavonoid-rich foods are needed to monitor diets. Monitoring diets and associating dietary patterns with health risks and outcomes is an essential step in the research process. The goal of this study is to determine the time- and dose-dependent formation of novel flavonoid metabolites in urine and plasma samples by conducting a randomized crossover trial of two dietary interventions with low (\~140 mg flavonoids/day) and high (\~420 mg flavonoids/day) flavonoid diets among healthy individuals. Foods with high or low flavonoid content will be incorporated into part…
Eligibility
- Age range
- 25–70 years
- Sex
- All
- Healthy volunteers
- Yes
Inclusion Criteria: * 1\. Age 25-70 years 2. BMI between 18 and 30 3. Have no known allergy to test food 4. have no known allergies to green tea, apples, blueberries, soy milk, and pineapples 5. Be not engaging in high-intensity exercise 6. Having blood and urine clinical tests in the normal range. 7. Be not taking antibiotics for six months 8. Be not currently taking medication 1. No taking any prescription drugs 2. If supplements were taken, flavonoid-enriched supplements (see list below) should be avoided. Major brands Notes Soy Isoflavones All flavonoid-enriched supplements sho…
Interventions
- Dietary SupplementHigh-Flavonoid Diet
Participants will consume the high-flavonoid diet daily for 3 weeks. The diet includes 1 Red Delicious apple (approximately 160 g), freeze-dried whole blueberry powder (25 g), soy milk (240 mL), and decaffeinated Lipton green tea ( 2 g tea leaves), providing approximately 420 mg flavonoids per day.
- Dietary SupplementLow-Flavonoid Diet
Participants will consume the low-flavonoid diet daily for 3 weeks. The diet includes 1/3 of Red Delicious, 107 g of pineapple; 8.3 g of blueberry powder plus 16.7 g of blueberry placebo powder; 80 mL of soy milk plus 160 mL of 2% low-fat milk; and 0.67 g tea leaves, providing approximately 140 mg flavonoids per day.
Location
- North Carolina Agriculture and Technical State University/ NRI BuildingKannapolis, North Carolina