The University of Texas Health Science Center at San Antonio
In this study, the investigators will use euglycemic/hyperinsulinemic clamps, longitudinal skeletal muscle biopsies, and single nucleus ribonucleic acid sequencing (RNA-Seq) and assay for transposase-accessible chromatin using sequencing (ATAC- seq) to reveal cell type-specific molecular response quantitative trait loci (rQTLs) acutely regulated by insulin signaling and glucose uptake.
The study investigators seek to find the relationship between basal and insulin stimulated Quantitative trait loci (QTL's) by utilizing vastus lateralis muscle biopsies before and after a euglycemic/hyperinsulinemic clamp. Each participant will have a catheter placed into an antecubital vein for the infusion of all test substances. A second catheter will be inserted retrogradely into a vein on the dorsum of the hand, and the hand will be placed into a thermoregulated box heated to 45 to 50 degrees Celsius. All participants will receive an infusion of \[3-3H\]glucose. After a 2-h basal tracer e…
Inclusion Criteria: * Males and Females Age 18-75 * Body Mass Index (BMI) \> 18 * Normal glucose tolerance as defined by: Fasting blood sugar \< 100 mg/dL 2-hour Oral Glucose Tolerance Test (OGTT) \< 140mg/dL Stable weight over the past 3 months defined as \>±5% change in body weight * Hemoglobin A1c \< 5.7 * Creatinine \< 2 * Aspartate aminotransferase/Alanine transaminase (AST/ALT) \< 75 * Blood sodium 135 to 145 mEq/L * Blood potassium 3.6 to 5.2 mmol/L\[MH1.1\] Exclusion Criteria: * Urine albumin excretion\>300mg/day * Partial thromboplastin time (PTT) \>35International normalized rat…
80mU/m2\*min euglycemic-hyperinsulinemic clamp
Arctuva estimate
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